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λ zap ii hela cdna library  (Agilent technologies)


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    Structured Review

    Agilent technologies λ zap ii hela cdna library
    λ Zap Ii Hela Cdna Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%BB+zap+ii+hela+cdna+library/pmc00110720-77-9-25
    Average 90 stars, based on 1 article reviews
    λ zap ii hela cdna library - by Bioz Stars, 2026-10
    90/100 stars

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    Infection:

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: The hDBR1 cDNA was without its own promoter and was placed under control of the S.pombe ura4 + promoter. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Plasmid Description pKN200 hDBR1 in pBlueScript (from λ ZAP II library by in vivo excision) pKN201 hDBR1 PCR product in pCR R II-TOPO TA cloning vector pKN203 Nde I– Bam HI fragment of hDBR1 in pET28a pKN205 Eco RI– Bam HI fragment of hDBR1 in pRS314GU pKN206 Eco RI– Bam HI fragment of hDBR1 in pRS316GU pKN207 Eco RI– Bam HI fragment of hDBR1 in pJK210 pCR R II-TOPO TA cloning vector (Invitrogen) pET28a Escherichia coli expression vector (Novagen) pJK210 Schizosaccharomyces pombe integration vector (15) pRS314GU Saccharomyces cerevisiae expression vector (14) pRS316GU Saccharomyces cerevisiae expression vector (14) Open in a separate window caption a8 Plasmids used in this study table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Primer Sequence KBN19 5′-GGA AAA TAA CAC ATT AGG AAG T-3′ KBN20 5′-CCA AGT AAG TCA GGA GCA CTG G-3′ KBN55 5′-CCT AGT CTA GAG GAT CCA GTC TAG GTG CAA GAG TGA AAC-3′ KBN62 5′-ATA TGG AAT TCC ATA TGC GGG TGG CTG TGG CTG G-3′ Open in a separate window caption a8 Primers used in this study Human cDNA library screen A HeLa cDNA library was purchased from Stratagene. .. Escherichia coli strain XL-1 Blue was infected with the λ ZAP II HeLa cDNA library and screened by plaque hybridization as suggested by the manufacturer (Stratagene). ..

    cDNA Library Assay:

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: The hDBR1 cDNA was without its own promoter and was placed under control of the S.pombe ura4 + promoter. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Plasmid Description pKN200 hDBR1 in pBlueScript (from λ ZAP II library by in vivo excision) pKN201 hDBR1 PCR product in pCR R II-TOPO TA cloning vector pKN203 Nde I– Bam HI fragment of hDBR1 in pET28a pKN205 Eco RI– Bam HI fragment of hDBR1 in pRS314GU pKN206 Eco RI– Bam HI fragment of hDBR1 in pRS316GU pKN207 Eco RI– Bam HI fragment of hDBR1 in pJK210 pCR R II-TOPO TA cloning vector (Invitrogen) pET28a Escherichia coli expression vector (Novagen) pJK210 Schizosaccharomyces pombe integration vector (15) pRS314GU Saccharomyces cerevisiae expression vector (14) pRS316GU Saccharomyces cerevisiae expression vector (14) Open in a separate window caption a8 Plasmids used in this study table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Primer Sequence KBN19 5′-GGA AAA TAA CAC ATT AGG AAG T-3′ KBN20 5′-CCA AGT AAG TCA GGA GCA CTG G-3′ KBN55 5′-CCT AGT CTA GAG GAT CCA GTC TAG GTG CAA GAG TGA AAC-3′ KBN62 5′-ATA TGG AAT TCC ATA TGC GGG TGG CTG TGG CTG G-3′ Open in a separate window caption a8 Primers used in this study Human cDNA library screen A HeLa cDNA library was purchased from Stratagene. .. Escherichia coli strain XL-1 Blue was infected with the λ ZAP II HeLa cDNA library and screened by plaque hybridization as suggested by the manufacturer (Stratagene). ..

    Hybridization:

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: The hDBR1 cDNA was without its own promoter and was placed under control of the S.pombe ura4 + promoter. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Plasmid Description pKN200 hDBR1 in pBlueScript (from λ ZAP II library by in vivo excision) pKN201 hDBR1 PCR product in pCR R II-TOPO TA cloning vector pKN203 Nde I– Bam HI fragment of hDBR1 in pET28a pKN205 Eco RI– Bam HI fragment of hDBR1 in pRS314GU pKN206 Eco RI– Bam HI fragment of hDBR1 in pRS316GU pKN207 Eco RI– Bam HI fragment of hDBR1 in pJK210 pCR R II-TOPO TA cloning vector (Invitrogen) pET28a Escherichia coli expression vector (Novagen) pJK210 Schizosaccharomyces pombe integration vector (15) pRS314GU Saccharomyces cerevisiae expression vector (14) pRS316GU Saccharomyces cerevisiae expression vector (14) Open in a separate window caption a8 Plasmids used in this study table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Primer Sequence KBN19 5′-GGA AAA TAA CAC ATT AGG AAG T-3′ KBN20 5′-CCA AGT AAG TCA GGA GCA CTG G-3′ KBN55 5′-CCT AGT CTA GAG GAT CCA GTC TAG GTG CAA GAG TGA AAC-3′ KBN62 5′-ATA TGG AAT TCC ATA TGC GGG TGG CTG TGG CTG G-3′ Open in a separate window caption a8 Primers used in this study Human cDNA library screen A HeLa cDNA library was purchased from Stratagene. .. Escherichia coli strain XL-1 Blue was infected with the λ ZAP II HeLa cDNA library and screened by plaque hybridization as suggested by the manufacturer (Stratagene). ..



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